Ultrafast and absolute quantification of SARS-CoV-2 on food using hydrogel RT-LAMP without pre-lysisArticle Published on 2023-01-152022-11-16 Journal: Journal of hazardous materials [Category] SARS, 치료기술, [키워드] Absolute quantification acute respiratory syndrome amplification cold chain Cold chain food contact infection coronavirus faster Food industry hydrogel Hydrogel RT-LAMP isothermal amplification microbe offer POCT point-of-care quantification reproducibility reverse transcription RNA RNA extraction RT-LAMP SARS-CoV-2 sensitivity specificity stability Surveillance Ultrafast detection. virus [DOI] 10.1016/j.jhazmat.2022.130050 PMC 바로가기
Influenza and other respiratory viruses in children: prevalence and clinical featuresArticle Published on 2022-12-012022-11-15 Journal: European Journal of Clinical Microbiology & Infect [Category] 신종인플루엔자, 진단, 치료기술, [키워드] age ARI Asthma clinical feature clinical manifestation collected cough COVID-19 pandemic Epidemic Epidemics Epidemiology female H1N1 ICU Infection Influenza influenza A influenza B other respiratory virus Patient positive Prevalence prevalent real-time RT-PCR respiratory respiratory infection respiratory virus respiratory virus respiratory viruses rhinoviruses RNA extraction specimens study period surveillance. tested virus was performed [DOI] 10.1007/s10096-022-04515-3 PMC 바로가기
Evaluation of the impact of pre-analytical conditions on sample stability for the detection of SARS-CoV-2 RNAArticle Published on 2022-11-012022-11-15 Journal: Journal of virological methods [Category] SARS, 치료기술, 치료제, [키워드] collected Community condition conditions cycle threshold Demand Diagnosis diagnostics False negative increase in independent Laboratory MOST PBS postal service provide RNA RNA extraction RT-qPCR Sample Sample stability. SARS-CoV-2 SARS-CoV-2 E gene SARS-CoV-2 RNA sensitivity stability Swab temperature tested Transport media transported Variation Viral load VTM [DOI] 10.1016/j.jviromet.2022.114607 PMC 바로가기
Reverse Transcription-Loop-Mediated Isothermal Amplification-CRISPR-Cas13a Technology as a Promising Diagnostic Tool for SARS-CoV-2Article Published on 2022-10-262022-11-16 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome caused change clinical samples coronavirus coronavirus disease COVID-19 CRISPR-Ca CRISPR-Cas CRISPR-Cas13 cycle threshold deaths develop Diagnosis diagnosis. diagnostic Diagnostic method early diagnosis effort exhibit highlighting isothermal isothermal amplification maintain Molecular diagnosis nasopharyngeal sample Negative predictive value New coronavirus NPV pandemic performed persist Positive predictive value PPV Prevalence protocol reaction required researcher RNA extraction RNA molecules RT-LAMP SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus sensitivity Sensitivity and specificity single nucleotide single-stranded specificity technology viral loads [DOI] 10.1128/spectrum.02398-22 PMC 바로가기
SARS-CoV-2 Contamination of Ambulance Surfaces and Effectiveness of Routine Decontamination Procedure: A Classic Hygiene Lesson for A Novel PathogenArticle Published on 2022-10-212022-11-16 Journal: International Journal of Environmental Research an [Category] COVID19(2023년), SARS, 치료기술, [키워드] Ambulance bypassed classic condition Contamination COVID-19 COVID-19 emergency COVID-19 patient COVID-19 patients decontamination Effectiveness Efficacy emergency medical services (EMS) FIVE glove health authorities healthcare-associated infection (HAI) Infection investigated Italy lesson medical service medical-transport-associated infection (MTAI) molecular MONITOR novel oxygen Patient positive prehospital emergency medicine. RNA extraction routine RT-qPCR SARS-CoV-2 selected surface swabs tested Transport was performed with COVID-19 [DOI] 10.3390/ijerph192013646 PMC 바로가기
The Efficient and Practical virus Identification System with ENhanced Sensitivity for Solids (EPISENS-S): A rapid and cost-effective SARS-CoV-2 RNA detection method for routine wastewater surveillance고형물에 대한 감도가 향상된 효율적이고 실용적인 바이러스 식별 시스템(EPISENS-S): 일상적인 폐수 감시를 위한 빠르고 비용 효율적인 SARS-CoV-2 RNA 검출 방법Article Published on 2022-10-152022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] applicability centrifugation city collected Concentration confirmed COVID-19 case COVID-19 demonstrated Detection sensitivity efficient Epidemiology evaluated examined followed by heat heat-inactivated identification Japan magnitude Mild multiplex normalized pellet Pepper mild mottle virus plant primer Primers qPCR qPCR quantification qPCR. Quantification method quantified ranged reported reverse primer reverse primers RNA concentration RNA concentrations RNA extraction SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection sensitivity Solid subsequent Surveillance System target targets the SARS-CoV-2 Treatment virus wastewater Wastewater-based epidemiology [DOI] 10.1016/j.scitotenv.2022.157101 PMC 바로가기 [Article Type] Article
Evaluation of filter paper to transport oro/nasopharyngeal samples to detect SARS-CoV-2 by RT-qPCRRT-qPCR에 의한 SARS-CoV-2 검출을 위한 구강/비인두 샘플 수송을 위한 여과지의 평가Article Published on 2022-10-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] addition amplification approach carried clinical sample concentrations detect evaluate evaluated false-negative false-negative results feasible Filter Filter Paper generate impregnation Inconclusive Laboratory N1 and N2 positive result presenting qPCR reference method resource RNA extraction RT-qPCR SARS-CoV-2 SARS-CoV-2 detection specimen target Transport Transportation. Viral load [DOI] 10.1016/j.jviromet.2022.114587 PMC 바로가기 [Article Type] Article
COVID salivary diagnostics: A comparative technical studyCOVID 타액 진단: 비교 기술 연구Article Published on 2022-09-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 assumption can be used Care Chain Reaction coronavirus coronavirus disease Coronavirus disease 2019 COVID COVID-19 Delta diagnostic diagnostics direct methods eliminating envelope golden standard heat heat-released RNA high accuracy indicate individuals infected individual infected individuals Intervention Isolation mass mass surveillance molecular molecular diagnostics Mouthwash nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs noninvasive NPs Open reading frame open reading frame 1 open reading frame 1ab ORF1ab pandemic patients polymerase chain polymerase chain reaction promote remained reported respiratory restriction RNA RNA extraction robust RT-PCR Saliva Saliva. salivary sampling SARS-CoV-2 sensitivity severe acute respiratory syndrome Coronavirus specificity Surveillance the patient Viral viral gene viral genes virus spread while widespread [DOI] 10.1002/jmv.27883 PMC 바로가기 [Article Type] Article
Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)역전사 루프 매개 등온 증폭(RT-LAMP)을 사용한 SARS-CoV-2 면봉 샘플에 대한 임상 테스트Article Published on 2022-08-182022-09-11 Journal: BMC Infectious Diseases [Category] SARS, 진단, 치료기술, [키워드] 95% CI Care change clinical clinical testing Diagnosis diagnostic Efficiency end point equipment isothermal amplification Laboratory limit Nasopharyngeal swab Nasopharyngeal swab samples nucleic acid nucleic acid extraction pH sensitive indicator. phenol Phenol red positive reaction RNA extraction RNA extraction kit RT-LAMP SARS-CoV-2 SARS-CoV-2 swab sample sensitive indicator sensitivity specificity was used [DOI] 10.1186/s12879-022-07684-w PMC 바로가기 [Article Type] Article
Collection and detection of SARS-CoV-2 in exhaled breath using face mask안면 마스크를 사용하여 호기에서 SARS-CoV-2 수집 및 감지Article Published on 2022-08-182022-09-11 Journal: PLoS ONE [Category] SARS, 치료기술, 치료제, [키워드] addition breath collected Collection Cross-infection electrostatic examined expected face Face mask Face masks Genes Mask Masks Molecular tests Nasopharyngeal swab Nasopharyngeal swab samples noninvasive nucleic acid ORF1 ORF1ab pandemic PCR PCR assay PCR assays PCR test PCR tests positive Prevent PROTECT protocol quantity RCA RdRP reduce RNA extraction Rolling circle amplification SARS-CoV-2 SARS-CoV-2 test SARS-CoV-2 tests significantly subjects target gene Target genes Transmission utility virus virus virus detection was performed was selected [DOI] 10.1371/journal.pone.0270765 PMC 바로가기 [Article Type] Article